Research Assistant

Unlike humans that have limited tooth replacement, many other vertebrates are polyphyodonty, meaning they have continuous tooth replacement throughout their lifetime. The corn snake, Pantherophis guttatus, is an exceptional polyphyodont model organism used to study continuous tooth replacement. At King?s College London, we are fortunate to have a complete series of corn snake embryos, from when the eggs have been laid to when they hatch: 0-60 days post-oviposition. The aim of this project is to image tooth development and replacement patterns of the corn snake in 2D using histological and immunohistochemistry, and in 3D using microCT scanning combined with Lugol?s Iodine staining, a technique known as diceCT. Snake tissue will be histological sectioned, and undergo trichrome staining or immunohistochemistry to look at gene expression patterns. Snake tissue will also be stained with Lugol's Iodine and microCT scanned, and carry out digital segmentation in the program Amira to produce 3D models of resulting phenotypes. The research assistant will carry out a mix of these techniques, to help collect high quality data for publication. The research assistant will also benefit from being part of a vibrant and accomplished lab group, which will support their development of research and professional skills, including working in a team, participating in discussions, reviewing the literature, project planning, and problem-solving.

Bachelor's Degree in Science or similar

• Animal handling
• Histological sectioning
• Trichrome Staining
• Immunohistochemistry
• Knowledge of dental anatomy
• microCT scanning
• 3D modelling (Amira/Avizo software)
• Lab safety

To be a model for Year 2 and 3 MBBS students to perform transthoracic echocardiogram (TTE), pleural ultrasound and abdominal ultrasound. The session is supervised by healthcare professionals trained in ultrasound who will act as facilitators and tutors instructing on ultrasound technique. You will be required to lie on a couch for up to 2 hours with the torso and abdomen exposed so the students can practice performing ultrasound on your chest wall or abdomen. This task involves application of ultrasound gel to an ultrasound probe that will be positioned at key landmarks on your chest and abdomen to visualise the heart, lung and abdominal structures. The facilitator will demonstrate how to obtain images of your heart/lung/abdominal organs using surface landmarks on your torso and abdominal walls before allowing the students to operate the probe and practice obtaining the same images. Your two hour session consists of 4 shorter rotations of 30 minutes each with approximately 10 students per rotation.

This event takes place on Thursday 10th July and you are required from 3:30pm-5:30pm at Guy's Campus, Shepherd's House, Chantler Centre.

You are required to lie on a couch for 2 hours with short breaks. The couch will have the head supported and a gown/blanket available. The torso must be exposed to allow the ultrasound of your heart, lungs and abdomen.

No experience of skill is required

To be a model for Year 2 and 3 MBBS students to perform transthoracic echocardiogram (TTE), pleural ultrasound and abdominal ultrasound. The session is supervised by healthcare professionals trained in ultrasound who will act as facilitators and tutors instructing on ultrasound technique. You will be required to lie on a couch for up to 2 hours with the torso and abdomen exposed so the students can practice performing ultrasound on your chest wall or abdomen. This task involves application of ultrasound gel to an ultrasound probe that will be positioned at key landmarks on your chest and abdomen to visualise the heart, lung and abdominal structures. The facilitator will demonstrate how to obtain images of your heart/lung/abdominal organs using surface landmarks on your torso and abdominal walls before allowing the students to operate the probe and practice obtaining the same images. Your two hour session consists of 4 shorter rotations of 30 minutes each with approximately 10 students per rotation.

This event takes place on Thursday 10th July and you are required from 11am-1pm at Guy's Campus, Shepherd's House, Chantler Centre.

You are required to lie on a couch for 2 hours with short breaks. The couch will have the head supported and a gown/blanket available. The torso must be exposed to allow the ultrasound of your heart, lungs and abdomen.

No experience of skill is required